The specified host bacteria in liquid culture cultured in a suitable temperature, usually for 16 to 24 hours.
(2) 100 μl of phage stock solution with the host bacteria suspended liquid 300 μl uniformly mixed and let stand 15 minutes to infect.
The mixture inoculated to test tubes containing 10 ml of fresh liquid medium in shaking culture for approximately 8 to 24 hours at the appropriate temperature, incubation time varies according to the different phage.
The medium moved into the centrifuge tube and centrifuged at 10,000 rpm (5,000 g) for 10 minutes to precipitate the host bacteria.
The supernatant was moved to a new tube, 0.22μm pore size filter (filter) to remove residual bacteria, ie non-phage stock solution of host bacteria. However, phage stock solution was transparent, not directly to the naked eye to determine their proliferative effects, it should be determined titer to confirm the effectiveness of the proliferation of cultured
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