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Double-layer agar culture

Publié le 26 Juin 2012

The specified host bacteria in liquid culture cultured in a suitable temperature, usually for 16 to 24 hours.

(2) 100 μl of phage stock solution with the host bacteria suspended liquid 300 μl uniformly mixed and let stand 15 minutes to infect.

The mixture added to 5 ml cooled to 45 ℃, 0.7% agar medium and uniformly mixed immediately after the tile on the booing of 1.8% agar plate.

4 to be the tablet solidification moved to the appropriate temperature incubator, generally cultured for 24 hours, incubation time varies according to the different phage.

The same steps to create another bacteriophage-free control group.

Tablet with the control group (6) to develop good clarity, usually containing the phage of the tablet was to clarify the like, while the host bacteria-only control group was muddy like.

7 plates containing bacteriophage placThe specified host bacteria in liquid culture cultured in a suitable temperature, usually for 16 to 24 hours.

(2) 100 μl of phage stock solution with the host bacteria suspended liquid 300 μl uniformly mixed and let stand 15 minutes to infect.

The mixture added to 5 ml cooled to 45 ℃, 0.7% agar medium and uniformly mixed immediately after the tile on the booing of 1.8% agar plate.

4 to be the tablet solidification moved to the appropriate temperature incubator, generally cultured for 24 hours, incubation time varies according to the different phage.

The same steps to create another bacteriophage-free control group.

Tablet with the control group (6) to develop good clarity, usually containing the phage of the tablet was to clarify the like, while the host bacteria-only control group was muddy like.

7 plates containing bacteriophage placed in -20 ℃ for 4 to 5 hours at room temperature, remove and thaw.

The thawed liquid moved into a centrifuge tube and centrifuged at 10,000 rpm (5,000 g) for 10 minutes to precipitate the host bacteria.

The supernatant was moved to a new tube of 0.22 μm filter (filter) to remove residual bacteria, ie non-host bacteria, phage liquid.

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The thawed liquid moved into a centrifuge tube and centrifuged at 10,000 rpm (5,000 g) for 10 minutes to precipitate the host bacteria.

The supernatant was moved to a new tube of 0.22 μm filter (filter) to remove residual bacteria, ie non-host bacteria, phage liquid.

related articles:Basic fibroblast growth factor

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